DNA
Part:BBa_K2100066:Design
Designed by: Colleen Foley Group: iGEM16_MIT (2016-10-19)
pEXPR EGSH: mKate
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal BglII site found at 115
Illegal BamHI site found at 663 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1334
Illegal SapI.rc site found at 646
Illegal SapI.rc site found at 716
Design Notes
This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
We synthesized this part from entry vectors.